CellGraph AB3000B
Imaging system to detect low-level light emission in a single living cell by using a highly sensitive EM-CCD camera.Installation in One Week !!! For Only 131 500 $ 115 000 € |
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Capturing extremely weak bioluminescent signals in living cells
Gentaur Cellgraph AB-3000B is an imaging system developed to detect low-level light emission in a single living cell using a highly sensitive EM-CCD camera. The detection of low-level light emission has been achieved by employing an optical system with high condensing efficiency and a cooled EM-CCD camera with the highest level of absolute sensitivity. By using a temperature and CO2 gas concentration* control system and a humidifying unit, the atmosphere inside the sample holding chamber is the same as that of inside CO2 incubators. This incubation system enables long-term observation of cultured cells and tissue slices in the living state. The Cellgraph system also includes a color separation mechanism with built-in optical filters, enabling multicolor reporter gene assay using multiple luciferases. The accompanying analysis software makes it possible to measure the intensity of the light emitted from individual cells on the acquired images, and to collect continuous data of the bioluminescence intensity on a cell-by-cell basis. The compact design system blocks ambient light completely, so that a dark room is not required. Imaging conditions, such as the selection of filters, the adjustment of lighting, and the duration of imaging, as well as the condition of the sample chamber and the focusing, are fully controlled by a PC with dedicated software. The Cellgraph is an ideal imaging system to observe faint bioluminescence emitted by cells and tissue for an extended period of time while keeping them alive. * The CO2 gas injection unit is an optional accessory.
Specifications
Product Number AB-3000B
Product NameCellgraph
Cooled CCD CameraCCD type: Back-illuminated EMCCD
Active pixel: 512 x 512 pixel
Pixel size: 16 x 16μm
AD Resolution: 14/16 bit
Cooling temperature:
-80°C with an air-cooling system when the air temperature is 25°C, -90°C with a water-cooling system*
(when the water temperature is 25°C)
-85°C with an air-cooling system when the air temperature is 20°C, -100°C with a water-cooling system*
(when the water temperature is 16°C)
*The water-cooling unit is provided as an optional accessory.
Objective Lens4x (NA 0.53) (Other magnification lenses are also available as optional accessories)
StageX-Y-Z axis manual stage, Z-axis motorized stage
Culture dish35mm culture dish
Constant temperature function(Room temperature +5°C) to 45°C, 0.1°C step
LightingWhite LED with dimming function Blue LED with dimming function
Optical FilterUp to three filters can be set (525LP/560 LP/620 LP are standard equipment)
Exposure time30 milliseconds to 90 minutes
Imaging intervalIndefinite
Control softwareOS:Windows 7/Vista/XP
System requirementRAM: 1 GB or more (512 MB or more for XP)
Hard Drive: 30 GB or more free disk space required
Interface: Full size PCI slot x1, serial port x1, USB port x1
Power (main body)AC100-240V 106VA (varies according to the components of the whole system)
Dimensions (main body)430 mm (W) x 600 mm (D) x 650 mm (H)
Weight (main body)Approx. 40kg
Optional Accessories
CCD camera water-cooling unitConstant-temperature water circulating system, heat-resistant tube for circulation
CO2 gas injection unitCO2 gas-mixer
CO2 gas humidifying unitBubbling system for gas humidification
Perfusion culture chamber unitChamber for perfusion
Objective lenses2x, 10x, 20x, 40x, 60x
Product NameCellgraph
Cooled CCD CameraCCD type: Back-illuminated EMCCD
Active pixel: 512 x 512 pixel
Pixel size: 16 x 16μm
AD Resolution: 14/16 bit
Cooling temperature:
-80°C with an air-cooling system when the air temperature is 25°C, -90°C with a water-cooling system*
(when the water temperature is 25°C)
-85°C with an air-cooling system when the air temperature is 20°C, -100°C with a water-cooling system*
(when the water temperature is 16°C)
*The water-cooling unit is provided as an optional accessory.
Objective Lens4x (NA 0.53) (Other magnification lenses are also available as optional accessories)
StageX-Y-Z axis manual stage, Z-axis motorized stage
Culture dish35mm culture dish
Constant temperature function(Room temperature +5°C) to 45°C, 0.1°C step
LightingWhite LED with dimming function Blue LED with dimming function
Optical FilterUp to three filters can be set (525LP/560 LP/620 LP are standard equipment)
Exposure time30 milliseconds to 90 minutes
Imaging intervalIndefinite
Control softwareOS:Windows 7/Vista/XP
System requirementRAM: 1 GB or more (512 MB or more for XP)
Hard Drive: 30 GB or more free disk space required
Interface: Full size PCI slot x1, serial port x1, USB port x1
Power (main body)AC100-240V 106VA (varies according to the components of the whole system)
Dimensions (main body)430 mm (W) x 600 mm (D) x 650 mm (H)
Weight (main body)Approx. 40kg
Optional Accessories
CCD camera water-cooling unitConstant-temperature water circulating system, heat-resistant tube for circulation
CO2 gas injection unitCO2 gas-mixer
CO2 gas humidifying unitBubbling system for gas humidification
Perfusion culture chamber unitChamber for perfusion
Objective lenses2x, 10x, 20x, 40x, 60x
Applications
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Features
- Acquire images of culturing cells or tissue samples on a 35 mm dish in the living state.
- Provide a CO2 incubator equivalent atmosphere by controlling the temperature, CO2 gas concentration and humidifying.
- Highly sensitive and accurate detection of faint bioluminescence with an EM-CCD camera.
- Isolates and captures multicolor faint bioluminescence.
- Easy operation via a user-friendly interface.
- Full control from a PC with dedicated software.
- Completely lightproof,compact design.